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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

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Sharma A, Kaur P, Kumar V, Gill KD

A study was conducted in South Korea with 41 young women with mild depressive disorder and 43 healthy controls

This is strongly recommended especially if you are going for a surgical treatment, as your doctor will be able to explain the timeline pre and post-procedure

5H, I), while overexpression of a codon-optimized L2HGDH (L2HGDH OPT ), which is resistant to the designed sgRNA, reverses this effect (Fig